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KMID : 0545120140240070943
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Journal of Microbiology and Biotechnology
2014 Volume.24 No. 7 p.943 ~ p.953
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Novel Alkali-Tolerant GH10 Endo-β-1,4-Xylanase with Broad Substrate Specificity from Microbacterium trichothecenolyticum HY-17, a Gut Bacterium of the Mole Cricket Gryllotalpa orientalis
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Kim Do-Young
Shin Dong-Ha
Jung So-Ra
Kim Hyang-Mi
Lee Jong-Suk
Cho Han-Young
Bae Kyung-Sook
Sung Chang-Keun
Rhee Young-Ha
Son Kwang-Hee
Park Ho-Yong
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Abstract
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The XylH gene (1,167-bp) encoding a novel hemicellulase (41,584 Da) was identified from the genome of Microbacterium trichothecenolyticum HY-17, a gastrointestinal bacterium of Gryllotalpa orientalis. The enzyme consisted of a single catalytic domain, which is 74% identical to that of an endo-β-1,4-xylanase (GH10) from Isoptericola variabilis 225. Unlike other endo-β- 1,4-xylanases from invertebrate-symbiotic bacteria, rXylH was an alkali-tolerant multifunctional enzyme possessing endo-β-1,4-xylanase activity together with β-1,3/β-1,4- glucanase activity, which exhibited its highest xylanolytic activity at pH 9.0 and 60oC, and was relatively stable within a broad pH range of 5.0-10.0. The susceptibilities of different xylosebased polysaccharides to the XylH were assessed to be as follows: oat spelts xylan > beechwood xylan > birchwood xylan > wheat arabinoxylan. rXylH was also able to readily cleave p-nitrophenyl (pNP) cellobioside and pNP-xylopyranoside, but did not hydrolyze other pNP-sugar derivatives, xylobiose, or hexose-based materials. Enzymatic hydrolysis of birchwood xylan resulted in the product composition of xylobiose (71.2%) and xylotriose (28.8%) as end products.
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KEYWORD
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endo-β-1, 4-xylanase, GH10 enzyme, Microbacterium trichothecenolyticum HY-17, mole cricket, gut bacterium
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